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APC Annexin V
APC Annexin V from BioLegend
(2)
Write a review
Citations:
(140)
Supplier Page
Supplier Page from
BioLegend for
APC Annexin V
Description
APC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
APC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640920
Quantity
100 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag
APC
NCBI Full Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (140)
(41)
Tofacitinib overcomes an IFNγ-induced decrease in NK cell-mediated cytotoxicity via the regulation of immune-related molecules in LC-2/ad
Thoracic cancer
March 1, 2021
Okita R, Shimizu K, Nojima Y, Saisho S, Nakata M.
NK cells
was determined by
flow cytometry
on Annexin V
staining
with allophycocyanin‐labeled Annexin V (#
640920
;
BioLegend
) according to the manufacturer's instructions. The percentage of apoptotic
NK cells
was calculated. Statistics Differences
(42)
The serine hydroxymethyltransferase-2 (SHMT2) initiates lymphoma development through epigenetic tumor suppressor silencing
Nature Cancer
February 11, 2021
Sara Parsa et al.
manufacturer. Cell death
assay
(annexin V
assay
). Cells were stained with
APC-annexin V
(catalog no.
640920
;
BioLegend
) and 7-aminoactinomycin D (7-ADD) as recommended by the manufacturer. Analysis was performed
(43)
Histone methyltransferase DOT1L controls state‐specific identity during B cell differentiation
EMBO reports
February 3, 2021
Muhammad Assad Aslam et al.
incubated for 20 min at room temperature in the dark. Following annexin V (
Biolegend
, catalog number
640920
)
staining
, the cells were washed once with Binding buffer and resuspended in Binding buffer containing
(44)
Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles
Nature communications
January 19, 2021
Wei Z, Zhang X, Yong T, Bie N, Zhan G, Li X, Liang Q, Li J, Yu J, Huang G,
et al
.
cat. No304006,
clone
HI30, 1/20 dilution) negative and stained with anti-Annexin V (
Biolegend
, cat. No
640920
, 1/20 dilution) and PI to analyze the apoptosis. For TAM analysis, cell suspensions were stained with
(45)
The inflammatory kinase IKKα phosphorylates and stabilizes c-Myc and enhances its activity
Molecular cancer
January 18, 2021
Moser B, Hochreiter B, Basílio J, Gleitsmann V, Panhuber A, Pardo-Garcia A, Hoesel B, Salzmann M, Resch U, Noreen M,
et al
.
"
Cell Signaling Technology
, Frankfurt, Germany",Cat#:
7074
; RRID: AB_2099233
APC Annexin V
(
1:50
),"
BioLegend
, CA, USA",Cat#:
640920
c-Myc/N-Myc (D3N8F) Rabbit mAb
(
1:1000
),"
Cell Signaling Technology
, Frankfurt, Germany",Cat#:
13987
;
(46)
Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells
Cell metabolism
January 5, 2021
Zhu XG, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf BN, Yamaguchi N, Arab A, Tavora B, Timson R,
et al
.
,
Thermo Fisher
,
11456D
Recombinant Mouse IFN-γ,
BioLegend
,
575306
Recombinant Human IFN-γ,
BioLegend
,
570206
APC Annexin V
,
BioLegend
,
640920
DAPI
,
Thermo Fisher
,
D1306
Zombie
NIR
Fixable Viability Dye,
BioLegend
,
423106
Recombinant Mouse TNF-α,
BioLegend
,
575202
Recombinant
(47)
Dietary Inorganic Nitrate Protects Hepatic Ischemia-Reperfusion Injury Through NRF2-Mediated Antioxidative Stress
Frontiers in pharmacology
January 1, 2021
Li S, Jin H, Sun G, Zhang C, Wang J, Xu H, Zhang D, Wang S.
United States) and anti-Ly6G (
ab122501
,
Abcam
, MA, United States) was bought from Abcam.
APC-Annexin V
(
640920
,
BioLegend
, CA, United States) was purchased from BioLegend.
Mouse
HIRI Model A
mouse
HIRI model was
(48)
Cross-resistance of cisplatin selected cells to anti-microtubule agents: Role of general survival mechanisms
Translational oncology
January 1, 2021
Patel RP, Kuhn S, Yin D, Hotz JM, Maher FA, Robey RW, Gottesman MM, Horibata S.
(49)
Concurrent Mutations in STK11 and KEAP1 Promote Ferroptosis Protection and SCD1 Dependence in Lung Cancer
Cell reports
December 1, 2020
Wohlhieter CA, Richards AL, Uddin F, Hulton CH, Quintanal-Villalonga À, Martin A, de Stanchina E, Bhanot U, Asher M, Shah NS,
et al
.
resuspended in 100uL of
Annexin V binding buffer
supplemented with 4uL of Annexin V-APC (
BioLegend
,
640920
) and incubated at room temperature for 15 minutes in the dark. After
incubation
, 200uL of
Annexin V binding buffer
(50)
Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy
Cell
November 25, 2020
Jiang L, Wang YJ, Zhao J, Uehara M, Hou Q, Kasinath V, Ichimura T, Banouni N, Dai L, Li X,
et al
.
Granzyme B inhibitor I
,Calbiochem,368050 Puromycin,
InvivoGen
,
ant-pr-1
G418,
InvivoGen
,
ant-gn-1
Blasticidin,InvivoGen,
ant
-bl-05 DAPI,
Vector Laboratories
,
H-1800-10
APC Annexin V
,
Biolegend
,
640920
Fixable Viability Dye eFluor™ 780,
Thermo Fisher
,
65-0865-14
West Dura chemiluminescent substrates,
Thermo Fisher
(51)
Impact of Erythropoietin Production by Erythroblastic Island Macrophages on Homeostatic Murine Erythropoiesis
International journal of molecular sciences
November 25, 2020
Perron-Deshaies G, St-Louis P, Romero H, Scorza T.
cat#
113806
), anti-Ter119 PE (
clone
TER119
,
Biolegend
, cat#
116208
), 7AAD and Annexin V (
Biolegend
, cat#
640920
conjugated with Allophycocyanin (APC) were used. Cells were stained on ice and protected from light
(52)
Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition
Nature immunology
October 1, 2020
Inclan-Rico JM, Ponessa JJ, Valero-Pacheco N, Hernandez CM, Sy CB, Lemenze AD, Beaulieu AM, Siracusa MC.
cultures. Cell viability was evaluated by 7-AAD (
Biolegend
Cat #
420403
) and Annexin V (
Biolegend
Cat #
640920
) double
negative staining
. Supernatant levels of IL-5 and IL-13 were then evaluated by
ELISA
. For intracellular
(53)
Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma
eLife
September 29, 2020
Cheng CC, Wooten J, Gibbs ZA, McGlynn K, Mishra P, Whitehurst AW.
(54)
Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation
Molecular therapy. Nucleic acids
September 4, 2020
Festag J, Thelemann T, Schell M, Raith S, Michel S, Jaschinski F, Klar R.
(55)
Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice
Allergy
August 1, 2020
Hirata SI, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, Shibuya N, Saito E, Adachi J, Abe Y,
et al
.
(56)
Consumption of the Fish Oil High-Fat Diet Uncouples Obesity and Mammary Tumor Growth through Induction of Reactive Oxygen Species in Protumor Macrophages
Cancer research
June 15, 2020
Liu L, Jin R, Hao J, Zeng J, Yin D, Yi Y, Zhu M, Mandal A, Hua Y, Ng CK,
et al
.
inhibitor
N-acetyl-L-cysteine
(NAC, A7250) was purchased from
Sigma-Aldrich
. APC conjugated Annexin-V (
640920
) was purchased from
Biolegend
. Antibodies used
for flow cytometry
were obtained from Biolegend and BD
(57)
Repression of the Type I Interferon Pathway Underlies MYC- and KRAS-Dependent Evasion of NK and B Cells in Pancreatic Ductal Adenocarcinoma
Cancer discovery
June 1, 2020
Muthalagu N, Monteverde T, Raffo-Iraolagoitia X, Wiesheu R, Whyte D, Hedley A, Laing S, Kruspig B, Upstill-Goddard R, Shaw R,
et al
.
Annexin binding buffer (10mM HEPES pH 7.40, 140mM NaCl, 2.5mM CaCl2) and 2μl of Annexin V-APC (
Biolegend
640920
) were added to the pellet and incubated for 15mins. Propidium iodide (PI, 10μg/ml) was added immediately
(58)
PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patients
The Journal of clinical investigation
June 1, 2020
Pothlichet J, Rose T, Bugault F, Jeammet L, Meola A, Haouz A, Saul F, Geny D, Alcami J, Ruiz-Mateos E,
et al
.
(59)
CXCR4 Signaling Has a CXCL12-Independent Essential Role in Murine MLL-AF9-Driven Acute Myeloid Leukemia
Cell reports
May 26, 2020
Ramakrishnan R, Peña-Martínez P, Agarwal P, Rodriguez-Zabala M, Chapellier M, Högberg C, Eriksson M, Yudovich D, Shah M, Ehinger M,
et al
.
AB_2783045 Anti-
Ki67
,
Thermo Fisher Scientific
,Cat#
11–5698-80
; RRID: AB_11151689 Annexin V - APC,
Biolegend
,Cat#
640920
,, Bacterial and
Virus
Strains
,Bacterial and
Virus
Strains
,Bacterial and
Virus
Strains
,, pLKO5d.EFS.SpCas9.P2A.PAC,"Heckl
(60)
The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice
Scientific reports
April 24, 2020
Lei W, Duan R, Li J, Liu X, Huston A, Boyce BF, Yao Z.
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Reviews
Write a Review
(1)
8/6/2018
Annexin V APC for Determining Apoptosis in Cells
Suzanne Tomchuck
We often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the...
Read Review
(1)
2/24/2017
Staining T Cells With Annexin V APC
Adam Guess
This reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ...
Read Review
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APC Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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